Rabbit anti Sheep IgM (heavy and light chains), conjugated with FITC

  • Catalog number
    MBS571483
  • Price
    Please ask
  • Size
    1 mL
  • Products_type
    Secondary Antibody
  • Products_short_name
    [Rabbit anti Sheep IgM (heavy and light chains)]
  • Clonality
    Polyclonal
  • Reactivity
    Sheep
  • Specificity
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Sheep IgM, heavy and light chains.
  • Storage_stability
    Store at 4 degree C.
  • Tested_application
    ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • Conjugation
    Anti-FITC Antibody
  • About
    Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    heavy   light   chains   conjugated   with  
  • Short name
    Rabbit anti IgM (heavy light chains), conjugated with FITC
  • Technique
    Rabbit, light, anti, FITC, IgM, antibody to, antibody Conjugates, Fluorescein, IgMs
  • Host
    Rabbit, Rabbits
  • Isotype
    IgM, IgM
  • Label
    FITC
  • Species
    Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
  • Alternative name
    production species: rabbit antibody to ovine Immunoglobulin M (heavy and light chains), coupled including fluorecein
  • Alternative technique
    rabbit-anti, antibodies, fluorescine, immunoglobulin-m
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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