MHC Class 2, I-A (FITC) Antibody
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Catalog numberGEN632859
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PricePlease ask
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Size100ug
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Also known asMHC Class 2, I-A
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Other namesMhc class I A; N/A; Mhc class I A; N/A; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameorla-uaa; N/A; N/A; N/A
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Gene name synonimsorla-uaa; N/A; N/A; N/A
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Other gene namesorla-uaa; N/A; N/A; N/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1
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Clone3H435
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Host organismMouse (Mus musculus)
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Source organismRat (Rattus norvegicus)
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Species reactivityMouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityRecognizes a polymorphic determinant of rat Ia antigen present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes la antigens on B-cells, dendritic cells and certain epithelial cells (1, 3, 4). It is particularly useful for distinguishing la positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodPurified IgG.
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Form AppearanceSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
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ConcentrationN/A
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Storage and shippingStore the antibody at -20 degrees Celsius.
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Tested applicationsFlow Cytometry (FC/FACS)
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolNT5C1A, FABP6, INSM1, RRN3, GATD3, INSM2, PTPRN, HLA-A, IGHV5-10-1, CIDEA
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Short nameMHC Class 2, I-A (FITC) Antibody
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameAnti-MHC Class 2, I-A (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene name5'-nucleotidase, cytosolic IA
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-04-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5'-nucleotidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefatty acid binding protein 6
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Synonyms gene name
- fatty acid binding protein 6, ileal
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fatty acid binding protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameINSM transcriptional repressor 1
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Synonyms gene name
- insulinoma-associated 1
- insulinoma associated 1
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Synonyms
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Locus
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Discovery year1995-01-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SNAG transcriptional repressors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRRN3 homolog, RNA polymerase I transcription factor
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Synonyms gene name
- RRN3 RNA polymerase I transcription factor homolog (yeast)
- RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-08-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglutamine amidotransferase class 1 domain containing 3
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Synonyms gene
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Synonyms gene name
- chromosome 21 open reading frame 33
- glutamine amidotransferase like class 1 domain containing 3A
- glutamine amidotransferase class 1 domain containing 3A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-05-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Glutamine amidotransferase class 1 domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameINSM transcriptional repressor 2
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Synonyms gene name
- insulinoma-associated 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-01-14
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
- SNAG transcriptional repressors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type N
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Synonyms
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Synonyms name
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Locus
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Discovery year1995-02-17
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Entrez gene record
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Pubmed identfication
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Classification
- Protein tyrosine phosphatases receptor type
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data