MHC Class 2, I-A (FITC) Antibody

  • Catalog number
    GEN632859
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    MHC Class 2, I-A
  • Other names
    Mhc class I A; N/A; Mhc class I A; N/A; N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    orla-uaa; N/A; N/A; N/A
  • Gene name synonims
    orla-uaa; N/A; N/A; N/A
  • Other gene names
    orla-uaa; N/A; N/A; N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG1
  • Clone
    3H435
  • Host organism
    Mouse (Mus musculus)
  • Source organism
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Recognizes a polymorphic determinant of rat Ia antigen present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes la antigens on B-cells, dendritic cells and certain epithelial cells (1, 3, 4). It is particularly useful for distinguishing la positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    Purified IgG.
  • Form Appearance
    Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
  • Concentration
    N/A
  • Storage and shipping
    Store the antibody at -20 degrees Celsius.
  • Tested applications
    Flow Cytometry (FC/FACS)
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • French translation
    anticorps
  • Gene target
    MHC   Class   I-A  
  • Gene symbol
    NT5C1A, FABP6, INSM1, RRN3, GATD3, INSM2, PTPRN, HLA-A, IGHV5-10-1, CIDEA
  • Short name
    MHC Class 2, I-A (FITC) Antibody
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
  • Label
    FITC
  • Alternative name
    Anti-MHC Class 2, I-A (FITC)
  • Alternative technique
    antibodies, fluorescine
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    INSM transcriptional repressor 2
  • Synonyms gene name
    • insulinoma-associated 2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2003-01-14
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Zinc fingers C2H2-type
    • SNAG transcriptional repressors
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 5-10-1
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
    • immunoglobulin heavy variable 5-A (provisional)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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