B-Raf Antibody
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Catalog numberGEN603123
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PricePlease ask
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Size100ug
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Also known asB-Raf
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Other namesB-Raf protein; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameN/A
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1,k
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Clone6A11
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Host organismMouse (Mus musculus)
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Source organismHuman (Homo sapiens)
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Species reactivityHuman (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivitySpecific for human B-Raf. On Western blots, it identifies a band at 105kD in human Jurkat cell lysates, while the antibody detected a 95kD band in mouse NIH-3T3 cell lysates. Reactivity with other related proteins has not been observed. Western blot utility has been confirmed with Jurkat and NIH-3T3 cell lysates. Based on amino acid sequence homology, this antibody is also expected to react with rat B-Raf.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodAffinity Purified by Protein A chromatography
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Form AppearancePurified by PBS, pH 7.4, containing 0.1% sodium azide
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ConcentrationN/A
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Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applicationsELISA (EL/EIA), Western Blot (WB)
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBRAF, BRAFP1
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Short nameB-Raf Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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Alternative nameAnti-B-Raf
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameB-Raf proto-oncogene, serine/threonine kinase
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Synonyms gene name
- v-raf murine sarcoma viral oncogene homolog B
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinase kinase kinases
- RAF family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameBRAF pseudogene 1
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma viral oncogene homolog B pseudogene 2
- v-raf murine sarcoma viral oncogene homolog B pseudogene 1
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Synonyms
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Locus
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Discovery year2003-07-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data