DR3/Apo3 Antibody
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Catalog number3057R-100
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PricePlease ask
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Size100 µg
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Antibody TargetDR3/Apo3
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Target Alternative NameWAL-1, APO-3, TRAMP, LARD
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acids 378 of mouse DR3
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AccessionAAH17526
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Gene Id85030
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) antigen affinity-purified rabbit anti-DR3/Apo3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot and Immunohistochemistry
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Application And UsagesWestern blot analysis (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually.
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DescriptionApoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily throμgh interaction with their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1, Apo3, TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1, overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD, TRAF2, and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis throμgh TRADD, FADD, and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL), thymus and spleen.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTNFRSF25
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Short nameDR3/Apo3 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameDR3/Apo3 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameTNF receptor superfamily member 25
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
- tumor necrosis factor receptor superfamily, member 25
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tumor necrosis factor receptor superfamily
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data