Mouse anti DGKa

  • Catalog number
    MUB2009S
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    Diacylglycerol kinase alpha is an enzyme that in Humans is encoded by the DGKA gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Tissue specificity: lymphocytes and oligodendroglial cells. Diacylglycerol kinases are involved in cell signaling, either as regulators of diacylglycerol levels or as intracellular signal-geneRating enzymes.
  • Antibody come from
    M5a is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with an Escherichia coli cell-expressed, affinity-purified glutathione S-transferase fusion protein of a C-terminal portion (part of the Catalytic domain) of Rat DGKu.
  • Other description
    Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
  • Clone
    M5a
  • Antigen antibody binding interaction
    Mouse anti DGKa Antibody
  • Antibody is raised in
    Mouse (Balb/c)
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    M5A is directed anti the C-terminal portion (part of the Catalytic domain) of Rat DGKa.
  • Research interest
    Cancer
  • Application
    Immunoprecipitation,Western blotting
  • Antibody s suited for
    M5A is useful for immunoprecipitation and western blot. Optimal antibody dilution should be determined by titration.
  • Storage
    Store at 4°C, or in small aliquots at –20°C.
  • Relevant references
    Brahim Houssa, Dick Schaap, Jose´ van der Wal, Kaoru Gotoi, Hisatake Kondoi, Akio Yamakawa, Masao Shibata, Tadaomi Takenawa, and Wim J. van Blitterswijk THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 272, No. 16, Issue of April 18, pp. 10422–10428, 1997. Santina Cutrupi. Src-mediated activation of -diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility. EMBO.2000, vol 19,46-13.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    DGKa  
  • Gene symbol
    DGKA
  • Short name
    Mouse anti DGKa
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG2a
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to diacylglycerol kinase, alpha 80kDa
  • Alternative technique
    murine, antibodies
  • Alternative to gene target
    diacylglycerol kinase, alpha 80kDa, DAGK and DAGK1 and DGK-alpha, DGKA and IDBG-39397 and ENSG00000065357 and 1606, phospholipid binding, Plasma membranes, Dgka and IDBG-198283 and ENSMUSG00000025357 and 13139, DGKA and IDBG-636422 and ENSBTAG00000004018 and 506348
Gene info
  • Identity
  • Gene
  • Long gene name
    diacylglycerol kinase alpha
  • Synonyms gene
  • Synonyms gene name
    • diacylglycerol kinase, alpha (80kD)
    • diacylglycerol kinase, alpha 80kDa
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1991-08-21
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Diacylglycerol kinases
    • EF-hand domain containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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