Anti Human CD3/CD8/CD45 Antibody-FITC/PE/PerCP
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Catalog number
3F18PE145P1-50T
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Price
Please ask
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Size
50 TEST
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Description
Anti Human CD3/CD8/CD45 Antibody-FITC/PE/PerCP is a human antibody resulting from the mixture of three monclonal antibodies, this reagent was [33-2A3] FITC conjugated, [143-44] PE conjugated and [D3/9] PerCP conjugated, the product is reported for use in flow cytometric analysis for three color, this antibody reacts with antigen of CD3, CD8, and CD45 to identify mature T lymphocytes, and leucocytes.
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Conjugated
FITC/ PE/ PerCP
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Clone
33-2A3, 143-44, D3/9
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Application
Flow Cytomtry Method
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Genes name
CD3/CD8/CD45
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Formulatin
Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Expiration
After 1 year
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Storage
2-8°C, store in dark, do not freeze
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Remark
For Recherche Use Only.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
CD8A, PTPRCAP, PTPRC
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Short name
Anti CD3/CD8/CD45 Antibody-FITC/PE/PerCP
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Technique
anti human, Antibody, Percp, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Anti CD3/CD8/CD45 Antibody-FITC/PE/PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Immunostep.
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Isotype
IgG2a, IgG1,IgG1
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Label
FITC
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Species
Human, Humans
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Alternative name
Mouse-CD3/CD8/CD45 Human Antibody [33-2A3, 143-44, D3/9]-FITC/PE/PerCP
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Alternative technique
antibodies, antibodies-percp, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
CD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
Product images
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