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Immunogen
Synthetic peptide corresponding to the N-terminus of human WNK4 (AA1-15)
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Antibody s target
Human WNK4
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Antibody s full description
Rabbit Anti-Human WNK4 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
PHA 2B antibody, PHA2B antibody, PRKWNK 4 antibody, PRKWNK4 antibody, Protein kinase lysine deficient 4 antibody, Serine/threonine protein kinase WNK4 antibody, Serine/threonine-protein kinase WNK4 antibody, WNK lysine deficient protein kinase 4 antibody, Wnk4 antibody, WNK4_HUMAN antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 134.739 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1214 was sufficient for detection of WNK4 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Junction
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Tissue specificity
Expressed in kidney, colon and skin.
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Scientific context
WNK4 is a serine/threonine kinase with important roles in the regulation of electrolyte homeostasis, cell signalling, survival and proliferation. Its exact function in cancer is unknown.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_115763
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Gene number
65266
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Protein number
Q96J92
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
WNK4
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Short name
Rabbit Anti- WNK4 Polyclonal: ATTO 633
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 633
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman WNK lysine deficient protein kinase 4 polyclonal: ATTO 633
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
WNK lysine deficient protein kinase 4, WNK4 and IDBG-51675 and ENSG00000126562 and 102724304,65266, transferase activity, Cell surfaces, Wnk4 and IDBG-211994 and ENSMUSG00000035112 and 69847, WNK4 and IDBG-640438 and ENSBTAG00000019907 and 100849076
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