ACHE Antibody (NT)
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Catalog number6706-100
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PricePlease ask
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Size100 μl
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Antibody TargetACHE
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Target Alternative NameACHE; Acetylcholinesterase
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceThis ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human ACHE.
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AccessionP22303
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Gene IdACHE
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl of antibody in PBS with 0.09% (W/V) sodium azide
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PurificationThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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ApplicationWestern blot, IHC, IF, FACS
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Positive ControlWestern blot: Jurkat, Raji and Y79 cell lysate, IHC: human brain tissue, IF: NCI-H460 cells, FACS: NCI-H460 cells
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Application And UsagesWestern blot: ~1:1000, FACS: ~1:10–1:50, IHC: ~1:10-1:50, IF: 1:10-1:50.
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DescriptionAcetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions. Alternative mRNA splicing gives rise to three forms of AChE. It plays a role in neuronal apoptosis. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic subunits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedVerified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolACHE
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Short nameACHE Antibody (NT)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameacetylcholinesterase (antibody to-) (NT)
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Alternative techniqueantibodies
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Alternative to gene targetacetylcholinesterase, ACEE and ARACHE and N-ACHE and YT, ACHE and IDBG-32513 and ENSG00000087085 and 43, protein self-association, nuclei, Ache and IDBG-204838 and ENSMUSG00000023328 and 11423, ACHE and IDBG-640435 and ENSBTAG00000001139 and 540446
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Gene info
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Identity
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Gene
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Long gene nameacetylcholinesterase (Cartwright blood group)
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Synonyms gene
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Synonyms gene name
- acetylcholinesterase (YT blood group)
- acetylcholinesterase (Yt blood group)
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Synonyms name
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Locus
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Discovery year1989-06-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Blood group antigens
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data