Lactoferrin (Mouse) ELISA Kit

  • Catalog number
    E4727-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    A Sandwich ELISA kit for the quantitative measurement of Lactoferrin in mouse serum, plasma, culture media samples
  • Detection Method
    Absorbance (450 nm)
  • Species Reactivity
    Mouse
  • Applications
    the in vitro quantitative determination of Mouse LTF/LF concentrations in serum, plasma and other biological fluids., Coefficient of variation is < 10%, Detection Range: 62.50 - 4000 pg/ml, Sensitivity: 37.50 pg/ml, Specificity: This kit recognizes Mouse LTF/LF in samples. No significant cross-reactivity or interference between Mouse LTF/LF and analogues was observed.
  • Sample Type
    Plasma, Serum, Other biological fluids
  • Features Benefits
    Detection Range: 62.50 - 4000 pg/ml, Sensitivity: 37.50 pg/ml, Specificity: This kit recognizes Mouse LTF/LF in samples. No significant cross-reactivity or interference between Mouse LTF/LF and analogues was observed., Coefficient of variation is < 10%
  • Storage Conditions
    4ºC
  • Shipping Conditions
    Gel Pack
  • Shelf life
    6 months
  • Background
    Lactoferrin is a glycoprotein present in exocrine secretions and in the secondary granules of polymorphonuclear neutrophils. It is the iron-binding protein in milk. Lactoferrin is known to regulate intestinal iron absorption, and to participate in the defense against bacteria. BioVision’s Lactoferrin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Lactoferrin. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for mouse Lactoferrin and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of mouse LTF/LF. The concentration of mouse Lactoferrin in the samples can be calculated by comparing the OD of the samples to the standard curve.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Lactoferrin (Mouse) ELISA Kit
  • Technique
    ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Mouse, Mouses
  • Alternative name
    Lactoferrin (Mouse) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    murine, elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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