FITC-PEG-Biotin,5K

• Catalog number
  FL044041-5K
• Price
  Please ask
• Size
  100mg

• Product category
  Fluorescent PEG derivatives
• Availability and ordering
  Please contact Gentaur for the latest availability and to request a quote. For larger or bulk quantity a discount may apply
• Technical datasheet
  Please contact our technical support every day between 9 am to 6 pm local time to request the latest datasheet, certificate of analysis and MSDS
• Other products
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Biochempeg Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biochempeg FITC is highly sensitive to pH extremes.
• Conjugation
  Biotinylated, Anti-FITC Antibody

• Gene target
  PEG  
• Short name
  FITC-PEG- ,5K
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  fluorecein-PEG-biotinilated,5K
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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