MAP1LC3B (D23) polyclonal antibody

• Catalog number
  AP0762-100ul
• Price
  Please ask
• Size
  100ul

• Alternative Name
  Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II
• Immunogen
  A synthetic peptide corresponding to residues in Human MAP1LC3B.
• Swiss Prot
  Q9GZQ8
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC IF
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  MAP1LC3B (D23) polyclonal antibody detects endogenous levels of LC3-I and LC3-II protein.
• Usage
  Western blot (WB) analysis of MAP1LC3B (D23) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(20ug) Lane2:HEK293T transfected with pEGFP-C3-LC3A whole cell lysate(20ug) Lane3:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(20ug)
• BiowMW
  ~ 16-18 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MAP1LC3B   D23  
• Gene symbol
  MAP1LC3B
• Short name
  MAP1LC3B (D23) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  MAP1LC3B (D23) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:13352
• Gene
  MAP1LC3B
• Long gene name
  microtubule associated protein 1 light chain 3 beta
• Synonyms
  • ATG8F
• GenBank acession
  • AF087871
• Locus
  16q24.2
• Discovery year
  2002-09-16
• Entrez gene record
  81631
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000137654

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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