Protein A Magnetic Beads

• Catalog number
  6507-1
• Price
  Please ask
• Size
  1 ml

• Description
  Reusable Protein A conjugated magnetic beads with ≥25 mg/ml binding capacity for antibody purification from multiple sources. Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A (contains five IgG binding domain, BV catalog # 6500B) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.
• Product Highlights
  •CONTENTS- Supplied as a 50% slurry in PBS with 0.02% sodium azide. •TECHNICAL SPECIFICATIONS- Parameter Description: Support Characteristics- Paramagnetic, spherical, 6 % cross-linked agarose Ligand- Recombinant Protein A Particle Size- 75 – 150 µm Binding Capacity- Generally >25 mg human IgG/ml wet beads Working Temperature-Room temperature Storage Solution- PBS w/0.02% NaN₃ Storage Temperature-4 – 8 °C •FEATURES- Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies. High affinity for Fc region of IgG antibodies from a variety of species. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
• Storage Temp
  +4°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Conjugation
  Magnetic beads

• Gene target
  Protein  
• Gene symbol
  CENPA, VAPA, AKAP10
• Short name
  Protein A
• Technique
  All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Magnetic bead separation is a quick, efficient, clean process that scientists use to replace filtration, centrifugation and separation techniques. Magnetic beads and particles are used as carriers of antigens, antibodies, catalyzers, proteins and nucleic acids, enabling action on cells, bacteria, viruses and other biological entities.
• Label
  Magnetic
• Alternative name
  Protein A Magnetic particles

Gene info

• Identity
  HGNC:1851
• Gene
  CENPA
• Long gene name
  centromere protein A
• Synonyms gene name
  • centromere protein A (17kD)
  • centromere protein A, 17kDa
• Synonyms
  • CENP-A
  • CenH3
• Synonyms name
  • centromere-specific histone
  • histone H3-like centromeric protein A
• GenBank acession
  • U14518
• Locus
  2p23.3
• Discovery year
  1988-05-31
• Entrez gene record
  1058
• RefSeq identity
  • NM_001809
• Classification
  • Constitutive centromere associated network
  • H3 histones
• VEGA ID
  OTTHUMG00000097073

Gene info

• Identity
  HGNC:12648
• Gene
  VAPA
• Long gene name
  VAMP associated protein A
• Synonyms gene name
  • VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
  • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• Synonyms
  • hVAP-33
  • VAP-A
• Locus
  18p11.22
• Discovery year
  1999-03-19
• Entrez gene record
  9218
• Pubmed identfication
  • 9920726
  • 9657962
• VEGA ID
  OTTHUMG00000131603

Gene info

• Identity
  HGNC:368
• Gene
  AKAP10
• Long gene name
  A-kinase anchoring protein 10
• Synonyms gene name
  • A kinase (PRKA) anchor protein 10
• Synonyms
  • D-AKAP2
  • PRKA10
  • MGC9414
• Synonyms name
  • dual-specificity A-kinase anchoring protein 2
  • protein kinase A anchoring protein 10
  • mitochondrial A kinase PPKA anchor protein 10
• GenBank acession
  • AF037439
• Locus
  17p11.2
• Discovery year
  1999-09-16
• Entrez gene record
  11216
• Pubmed identfication
  • 9326583
• RefSeq identity
  • NM_007202
• Classification
  • A-kinase anchoring proteins
• VEGA ID
  OTTHUMG00000059515

MeSH Data

• Name
  Immunomagnetic Separation
• Concept
  Scope note: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
• Tree numbers
  • E02.095.437
  • E05.200.500.363.441
  • E05.242.363.441
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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