anti-SMAD3 Antibody

• Catalog number
  49-315
• Price
  Please ask
• Size
  0.05 mg

• Gene info
  SMAD3, a member of SMAD family of proteins, is involved in TGF-β signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-β and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia. Smad 3 (Mothers against decapentaplegic homolog 3), a transcriptional regulator, is activated by TGF-β and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.
• Gene names
  SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3
• Antibodie s label
  unlabelled serum
• Purity
  Protein G Column
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  SMAD3 antibody was made against a portion of amino acids 100 - 150 of SMAD3 (Human).
• Immuno application
  Western Blot, immuno-histo-chemistry
• Antibody advice
  SMAD3 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 ug/mL.
• Suspention pH azide
  PBS, 0.2% gelatin, 0.05% sodium azide.
• Keep at
  SMAD3 antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.
• Works with
  Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat
• Omim nr
  51338669
• Ncbi nr
  P84022
• Vial with antibody
  in solution
• NCBI GENE nr
  4088
• GENE symbol
  SMAD3
• Antigene name
  SMAD family member 3
• Source
  Homo sapiens
• Protein nr
  P84022
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SMAD3  
• Gene symbol
  SMAD3
• Short name
  anti-SMAD3 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toSMAD3 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

Gene info

• Identity
  HGNC:6769
• Gene
  SMAD3
• Long gene name
  SMAD family member 3
• Synonyms gene
  • MADH3
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3 (Drosophila)
• Synonyms
  • JV15-2
  • HsT17436
• GenBank acession
  • BC050743
• Locus
  15q22.33
• Discovery year
  1996-11-15
• Entrez gene record
  4088
• Pubmed identfication
  • 8774881
  • 8673135
• RefSeq identity
  • NM_005902
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000133230
• Locus Specific Databases
  • The Globin Gene Server

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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