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Clone
Pab 1801
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Immunogen
Fusion protein (Human), amino acids 46-53
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Antibody s full description
Mouse Anti-Human p53 Monoclonal IgG1 Antibody, Clone: Pab 1801: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
P53 Antibody, LFS1 Antibody, TRP53 Antibody, FLJ92943 Antibody, TP53 Antibody, p53 Tumor Suppressor Antibody, Phosphoprotein p53 Antibody, TP53 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor protein p53 Antibody
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Raised in
Mouse
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Antibody s target
p53
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Primary research fields
Cancer, Oxidative Stress, Cancer, Apoptosis, Cell Signaling, Epigenetics
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Brandname
None
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Antibodies applications
WB, IHC, IP, ELISA, RIA, FCM
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Antibody s reactivity
Human
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~53kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Nucleus , PML body , Endoplasmic Reticulum
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
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Bibliography
1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
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Released date
1-Jul-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_000537.3
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Gene number
7157
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Protein number
P04637
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-p53 Monoclonal Antibody, Clone Pab1801 (SMC-183). Tissue: esophagus cancer cells. Species: Human. Primary Antibody: Mouse Anti-p53 Monoclonal Antibody (SMC-183) at 1:50000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Western Blot analysis of Human HeLa cell lysates showing detection of p53 protein using Mouse Anti-p53 Monoclonal Antibody, Clone Pab1801 (SMC-183). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p53 Monoclonal Antibody (SMC-183) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-p53 Antibody [Pab1801] used in Immunohistochemistry (IHC) on Human esophagus cancer cells (SMC-183) Mouse Anti-p53 Antibody [Pab1801] used in Western Blot (WB) on Human HeLa cell lysates (SMC-183)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
