HER2, Active, Human Recombinant
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Catalog number
8011-50
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Price
Please ask
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Size
50 µg
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Synonyms
HER, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
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Alternative_names
HER, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
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Description
Active, Human Recombinant Her2 for functional assays.
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Recombinant
Yes
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Source
E. coli
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Activity Specifications test method
> 20 U/mg
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Biological activity
> 20 U/mg
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Results
> 20 U/mg
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Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of ADP per min. at 25°C, pH 7.2
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Molecular Weight
~ 100 kDa protein in SDS-PAGE under reducing conditions (aa 676 - end, N terminal-GST tag)
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
0.2 mg/ml
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Appearance
Liquid
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Physical form description
0.2 mg/ml in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
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Background Information
HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis. HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility.
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Amino acid sequence
aa 676 - end
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ERBB2
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Short name
HER2, Active, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
HER2, functionnal, H. sapiens Rec.
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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