OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit (CPD)

We send this reagant on

Room temperature
The temperatureerature for storage is

4ºC
Technical file

more kit.pdf
SDS or safty data sheet

details (STA-327) GHS.pdf
Type of reagent

Oxidative Stress/Damage
Related techniques

Oxidative Stress/ Damage, DNA / RNA Damage and Repair, UV Induced DNA Damage test kits
Use of the reagent

Our OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
Cellular use

Measure CPD structures of DNA lesions induced by UV light
Protocol information

Detect structures by immunofluorescence staining under a fluorescence microscope
Species compatible to

Humans
Use of this test kit or product

Genetics lab
Antigen

a specific protein sequence
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,67
Molecular weight

in kilo Danlto or kDa
This reagent is purified

0.97
Description

Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.

Gene target

OxiSelect™ Cellular DNA Damage Staining test kit CPD
Gene symbol

CPD, PRKDC, HLA-DOA, HOMER2
Short name

OxiSelect™ Cellular UV- DNA Damage Staining test kit (CPD)
Technique

cellular, dna
Alternative name

OxiSelect™ Cellular ultraviolet-Induced Desoxyribonucleic acid Damage coloring test reagent (CPD)
Alternative technique

tests, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

cellular