This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Trek-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
K2p2.1; KCNK2; MGC126742; MGC126744; TPKC1; TREK; TREK-1; TREK1; hTREK-1c; hTREK-1e
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~47 kDa Trek 1 of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Synthetic peptide corresponding to residues surrounding amino acids 417 of mouse Trek-1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Trek-1 and Trek-2 belong to the tandem-pore K+ channel family that has two pore-forming domains and four transmembrane segments. Trek-1 is expressed throμghout the central nervous system whereas Trek-2 is found mostly in the cerebellum, spleen and testis. Trek-1 is activated by arachidonic acid and polyunsaturated fatty acids.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.