CD86, B7-2, B-Cell activated, Monocyte, Clone: BU63, Mouse Monoclonal antibody-Human, FITC; flow

  • Catalog number
    OBT0391F
  • Price
    Please ask
  • Size
    0.1 mg
  • Alternative name 1
    CD86, B7-2, B-Cell activated, Monocyte, Clone: BU63, Mab anti-Human, FITC; flow
  • Alternative name 2
    CD86, B7-2, B-Cell activated, Monocyte, Clone: BU63, Mab antibody-Human, FITC; flow
  • Alternative name 3
    CD86, B7-2, B-Cell activated, Monocyte, Clone: BU63, Mab antibody-Homo sapiens, FITC; flow
  • Alternative name 4
    anti-CD86 B7-2 B-Cell activated Monocyte Clone: BU63 flow
  • Alternative name 5
    CD86 B7-2 B-Cell activated Monocyte Clone: BU63 flow
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    For cells, cell lines and tissues in culture till half confluency.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    CD86, CD276, HHLA2, RNY4P2, LRRC23, PCDHGB7, NCR3LG1, CD274, TLX1NB, CD80
  • Short name
    CD86, B7-2, B- activated, Monocyte, Clone: BU63, Mouse Monoclonal antibody- , FITC;
  • Technique
    Mouse monoclonal, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Label
    FITC
  • Species
    Human, Humans, Mouses
  • Alternative name
    CD86 molecule, B7-2, B-cellular activated, Monocyte, clonality: BU63, Mouse monoclonal (antibody to-)-H. sapiens, fluorecein; flow
  • Alternative technique
    antibodies, murine, fluorescine
  • Alternative to gene target
    CD86 molecule, B7-2 and B7.2 and B70 and CD28LG2 and LAB72, CD86 and IDBG-52336 and ENSG00000114013 and 942, coreceptor activity, Cell surfaces, Cd86 and IDBG-158071 and ENSMUSG00000022901 and 12524, CD86 and IDBG-633264 and ENSBTAG00000013118 and 414345
  • Tissue
    cell
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    RNY4 pseudogene 2
  • Synonyms gene name
    • RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 2
    • RNA, Ro-associated Y4 pseudogene 2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-08-25
  • Entrez gene record
  • RefSeq identity
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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