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Category
Primary Antibodies
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Long description
Cell Distribution: CD80 is rapidly induced on the surface of activated B cells, T cells or monocytes and is expressed at high levels on dendritic cells._x000B__x000B_Function: CD80 is one of two ligands (the other CD86) for CTLA4 and CD28. CD80 acts as co-stimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient costimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD86 in T helper cell differentiation.
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Antibody come from
Recombinant expressed extra-cellular domain of human CD80.
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Other description
Provided as 0.2µm sterile filtered solution in phosphate buffered saline. Protein A/G Chromatography
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Clone
AS29
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Antigen antibody binding interaction
Mouse anti CD 80 antibody B7.1 (extra-cellular domain) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Neutralization, Immunoprecipitation
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Antibody s suited for
Neutralization: AS29 has been shown to block the interaction of soluble hCD80 with hCTLA-4; Immunoprecipitation: This antibody has been used to immunoprecipitate >90% of CD80 in solution using a double antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol. 70, 266, 1980)
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Storage
-20ºC
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Relevant references
1. Dakappagari, N., et al. 'CD80 (B7.1) is expressed on both malignant B cells and nonmalignant stromal cells in non-Hodgkin lymphoma.' Cytometry B Clin Cytom 2012, 82, 112-119_x000B__x000B_2. Hall, K., et al. 'Defining the role of CD46, CD80 and CD86 in mediating adenovirus type 3 fiber interactions with host cells.' Virology 2009, 392, 222-229_x000B__x000B_3. Rau, F.C., et al. 'B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion.' J. Immunol. 2009, 183, 7661-7671
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
