Osteopontin, human recombinant

• Catalog number
  7335-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  OPN, SPP-1, BNSP, BSPI, ETA-1
• Alternative_names
  OPN, SPP-1, BNSP, BSPI, ETA-1
• Description
  Plays an important role in bone remodeling
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 microgram of protein
• Molecular Weight
  36.2 kDa (321 aa, 17-314 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, and 2 mM EDTA.
• Background Information
  Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Osteopontin  
• Gene symbol
  SPP1
• Short name
  Osteopontin, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Osteopontin, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11255
• Gene
  SPP1
• Long gene name
  secreted phosphoprotein 1
• Synonyms gene
  • BNSP
  • OPN
• Synonyms gene name
  • osteopontin
  • bone sialoprotein I
• Synonyms
  • BSPI
  • ETA-1
• Synonyms name
  • early T-lymphocyte activation 1
• Locus
  4q22.1
• Discovery year
  1989-03-06
• Entrez gene record
  6696
• Pubmed identfication
  • 1575754
• Classification
  • Receptor ligands
  • SIBLING family
• VEGA ID
  OTTHUMG00000130599

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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