Human CellExp™ LEPR/CD295, human recombinant

  • Catalog number
    7497-50
  • Price
    Please ask
  • Size
    50 ug
  • Synonyms
    LEPR, CD295, DKFZp686B1731, LEP-R, OB-R, OBR, Leptin receptor
  • Alternative_names
    LEPR, CD295, DKFZp686B1731, LEP-R, OB-R, OBR, Leptin receptor
  • Description
    Mediates the biological function of the adipocyte-specific hormone Leptin
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro-B cells transfected with rhLEPR-Fc. The ED50 for this effect is typically 0.015-0.13 ug/ml in the presence of 3 ng/ml rhLeptin.
  • Results
    Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro-B cells transfected with rhLEPR-Fc. The ED50 for this effect is typically 0.015-0.13 ug/ml in the presence of 3 ng/ml rhLeptin.
  • Molecular Weight
    This protein is fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 121 kDa expressed. The predicted N-terminus is Phe22. Protein migrates as 150-165 kDa in reduced SDS-PAGE resulting from glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Background Information
    Leptin receptor (LEPR), also known as LEP-R, cluster of differentiation 295 (CD295), OB-R and B219, is a single-transmembrane-domain receptor of the gp130 family of cytokine receptors. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this protein have been associated with obesity and pituitary dysfunction. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    LEPR
  • Short name
    CellExp™ LEPR/CD295, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ leptin receptor/CD295, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    leptin receptor, CD295 and LEP-R and LEPRD and OB-R and OBR, LEPR and IDBG-99469 and ENSG00000116678 and 3953, identical protein binding, Extracellular, Lepr and IDBG-167734 and ENSMUSG00000057722 and 16847, LEPR and IDBG-638266 and ENSBTAG00000005910 and 497205,785813
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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