Anti-phospho-Rsk1 (Thr359/Ser363), Rabbit Monoclonal Antibody

  • Catalog number
    A1132-50
  • Price
    Please ask
  • Size
    50 µl
  • Antibody Target
    RPS6KA1
  • Target Alternative Name
    rsk-1, rsk 1, Active RSK1, RSK1, RSK, human RSK1, active kinase, active kinases, recombinant RSK1, recombinant RSK, kinase activity assay, protein kinase
  • Antibody Type
    Monoclonal
  • Clone
    RM233
  • Species Reactivities
    Human
  • Immunogen Sequence
    A phospho-peptide corresponding to human phospho-Rsk1 (Thr359/Ser363)
  • Accession
    Q15418 , Q15349
  • Gene Id
    6195 , 6196
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
  • Purification
    Affinity purified using protein A
  • Application
    IHC, WB
  • Positive Control
    WB: A431 cell lysate; IHC: human cerebral cortex tissue
  • Application And Usages
    WB: 1:1000 - 1:2000 dilution; IHC: 1:200 -1:500 dilution
  • Description
    The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90(rsk). RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. Moller described the cloning and characterization of 3 genes encoding RSKs, and HU1 (also named RPS6KA1, or RSK1) cDNA encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. Northern blot and RNase protection analyses detected an approximately 3.5-kb HU1 transcript in lymphocytes, skeletal muscle, liver, and adipose tissue. Zeniou determined the expression of the RSK1, RSK2, and RSK3 genes in various human tissues, during mouse embryogenesis, and in mouse brain. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-phospho-Rsk1 (Thr359/Ser363), Rabbit Monoclonal Antibody
  • Technique
    Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibody tophosphorilated-Rsk1 (Thr359/Ser363), production species: rabbit monoclonal (antibody to-)
  • Alternative technique
    antibodies, rabbit-anti
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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