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Gene info
CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.
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Gene names
CDK9, C-2k, CDC2L4, CDC2-related kinase, Cell division protein kinase 9, Cyclin-dependent kinase 9, CDC2-related protein kinase, PITALRE, TAK, CTK1
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Antibodie s label
unlabelled serum
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Purity
Delipidation and Defibrination
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
CDK9 antibody was made against multiple synthetic peptides corresponding to C-Terminus of CDK9 (Human).
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno precipitation
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Antibody advice
CDK9 antibody can be used in Western Blot, and immunohistochemistry starting at 2.5 ug/mL.
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Suspention pH azide
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
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Keep at
Store CDK9 antibody at -20 grades C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 grades C or below. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human, Mouse, Rat
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Omim nr
68067660
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Ncbi nr
P50750
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Vial with antibody
in solution
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NCBI GENE nr
1025
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GENE symbol
CDK9
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Antigene name
cyclin-dependent kinase 9
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Source
Homo sapiens
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Protein nr
P50750
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
