VEGF165, human recombinant
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Catalog number
4363-50
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Price
Please ask
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Size
50 ug
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Synonyms
Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen
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Alternative_names
Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen
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Description
A signal protein that stimulates vasculogenesis and angiogenesis
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Biological activity
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.
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Results
1.0-7.0 ng/ml
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Molecular Weight
38.2 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized with no additives
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Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background Information
VEGF (Vascular Endothelial Growth Factor) is a secreted homodimeric protein, secreted by a variety of Vascularized tissues. VEGF stimulates endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
VEGF165, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
VEGF165, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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