Rabbit Anti-Human MAPKAPK3 (pTyr76) Polyclonal: Biotin
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Catalog numberSPC-1016D-BI
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PricePlease ask
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Tyr76 of human MAPKAPK3 (AA73-79)
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Antibody s targetHuman MAPKAPK3 (pTyr76)
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Antibody s full descriptionRabbit Anti-Human MAPKAPK3 (pTyr76) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other name3pK antibody, MAP kinase-activated protein kinase 3 antibody, MAPK activated protein kinase 3 antibody, MAPK3_HUMAN antibody, MAPKAP antibody, MAPKAP kinase 3 antibody, MAPKAP-K3 antibody, Mapkapk3 antibody, Mitogen activated protein kinase activated protein kinase 3 antibody, MK-3 antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 42.987 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1016 was sufficient for detection of MAPKAPK3 (pTyr76) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus, Cytoplasm
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Tissue specificityWidely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
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Scientific contextThe near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_004626
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Gene number7867
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Protein numberQ16644
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolMAPKAPK3
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Short nameRabbit Anti- MAPKAPK3 (pTyr76) Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman MAPKAPK3 (pTyr76) polyclonal: biotinilated
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameMAPK activated protein kinase 3
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Synonyms gene name
- mitogen-activated protein kinase-activated protein kinase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAPK activated protein kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data