Bacillus anthracis PA, Protective Antigen (Anthrax) (Biotin)

Other size

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Description

Antigens are peptides or recombinant or native dependent on the production method. Bacillus Anthracis and Cereus are the most known Bacilli. Anthrax has a very high toxin level and is used in Biologic warfare. However 1 is a harmless product.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Gene target

Bacillus anthracis Protective Anthrax
Short name

Bacillus anthracis PA, Protective Antigen (Anthrax) ( )
Technique

antigen, antigenes
Label

Biotin
Alternative name

Bacillus anthracis PA, Protective protein (Anthrax) (biotinilated)
Alternative technique

antigenes

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data