Human CellExp™ HAVCR1 / KIM1 / TIM1, human recombinant
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Catalog number7494-10
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PricePlease ask
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Size10 ug
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SynonymsHAVCR1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1, Hepatitis A virus cellular receptor 1
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Alternative_namesHAVCR1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1, Hepatitis A virus cellular receptor 1
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DescriptionMay be involved in the moderation of asthma and allergic diseases
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Biological activityMeasured by its ability to inhibit anti-CD3 induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/ml anti-CD3 and rhTIM1. The ED50 for this effect is typically 0.15-1.0 ug/ml.
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ResultsMeasured by its ability to inhibit anti-CD3 induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/ml anti-CD3 and rhTIM1. The ED50 for this effect is typically 0.15-1.0 ug/ml.
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Molecular WeightThis protein contains a poly histidine tag at C-terminus and has a calculated MW of 31.3 kDa after removal of signal peptide. In DTT-reduced SDS-PAGE, rhHAVCR1 protein migrates as 80-100 kDa poly peptide due to high glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationHepatitis A virus cellular receptor 1 also known as HAVCR1, HAVCR, KIM1, TIM, TIM1, TIMD1, is widely expressed with highest levels in kidney and testis. The protein encoded by HAVCR1 gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene. HAVCR1 may play a role in T-helper cell development, the regulation of asthma and allergic diseases and in kidney injury and repair. In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolHAVCR1, TIMELESS, ARHGEF5
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Short nameCellExp™ HAVCR1 / KIM1 / TIM1, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ hepatitis A virus cellular receptor 1 / KIM1 / TIM1, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targethepatitis A virus cellular receptor 1, HAVCR1 and IDBG-55259 and ENSG00000113249 and 26762, protein binding, Plasma membranes
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Gene info
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Identity
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Gene
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Long gene namehepatitis A virus cellular receptor 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametimeless circadian regulator
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Synonyms gene name
- timeless (Drosophila) homolog
- timeless homolog (Drosophila)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRho guanine nucleotide exchange factor 5
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Synonyms gene name
- Rho guanine nucleotide exchange factor (GEF) 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Dbl family Rho GEFs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
