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Synonyms
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
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Alternative_names
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
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Description
Contributes to the induction of non - cytocidal TNF effects
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥92%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 ug/mL (100 ul/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 ug/mL.
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Results
Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 ug/mL (100 ul/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 ug/mL.
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Ile 22. DTT-reduced Protein migrates as 30-35 kDa due to different glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Tumor necrosis factor receptor 1 (TNF-R1) also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial Hibernian fever (FHF).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF1A
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Short name
CellExp™ TNFR1 / CD120a / TNFRSF1A, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ TNFR1 / CD120a / tumor necrosis factor receptor superfamily, member 1A, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
tumor necrosis factor receptor superfamily, member 1A, CD120a and FPF and MS5 and p55 and p55-R and p60 and TBP1 and TNF-R and TNF-R-I and TNF-R55 and TNFAR and TNFR1 and TNFR1-d2 and TNFR55 and TNFR60, TNFRSF1A and IDBG-13807 and ENSG00000067182 and 7132, tumor necrosis factor binding, nuclei, Tnfrsf1a and IDBG-189844 and ENSMUSG00000030341 and 21937, TNFRSF1A and IDBG-640513 and ENSBTAG00000004211 and 282527
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