Anti-E3 ubiquitin ligase (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-9663R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideE3 ubiquitin ligase
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SpecificityThis antibody reacts specifically with E3 ubiquitin ligase
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsMitochondrion
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Antigen SourceKLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218
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Gene ID79594
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Swiss ProtQ969V5
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationExhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. The function may implicate its abilty to sumoylate DNM1L. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response. Can mediate DDX58 sumoylation and disrupt its polyubiquitination.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsGIDE; MAPL; MULAN; RNF218; C1orf166; Mitochondrial ubiquitin ligase activator of NFKB 1; E3 SUMO-protein ligase MUL1; E3 ubiquitin-protein ligase MUL1; Growth inhibition and death E3 ligase; Mitochondrial-anchored protein ligase; Putative NF-kappa-B-activating protein 266; RING finger protein 218; MUL1
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Also known asE3 ubiquitin ligase Polyclonal Antibody
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Other nameAnti-E3 ubiquitin ligase Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolITCH, HECTD3, PRKN
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Short nameAnti-E3 ubiquitin ligase
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameE3 ubiquitin ligase Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameitchy E3 ubiquitin protein ligase
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Synonyms gene name
- itchy (mouse homolog) E3 ubiquitin protein ligase
- itchy E3 ubiquitin protein ligase homolog (mouse)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-27
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Entrez gene record
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Pubmed identfication
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Classification
- C2 domain containing
- HECT domain containing
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHECT domain E3 ubiquitin protein ligase 3
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Synonyms gene name
- HECT domain containing E3 ubiquitin protein ligase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-07-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HECT domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameparkin RBR E3 ubiquitin protein ligase
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Synonyms gene
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Synonyms gene name
- Parkinson disease (autosomal recessive, juvenile) 2, parkin
- parkinson protein 2, E3 ubiquitin protein ligase (parkin)
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Synonyms
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Synonyms name
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Locus
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Discovery year1997-08-18
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Entrez gene record
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Pubmed identfication
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Classification
- RBR E3 ubiquitin ligases
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
- E05.393.620.311
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data