Mouse anti Cytokeratin 4 / Keratin K4

• Catalog number
  MUB0313S
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Antibody come from
  6B10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
• Other description
  Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
• Clone
  6B10
• Antigen antibody binding interaction
  Mouse anti Cytokeratin 4 / Keratin K4 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Canine,Feline,Human,Zebrafish
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stRatified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.
• Research interest
  Cancer,Cytoskeleton
• Application
  Flow Cytometry,Immunocytochemistry,Western blotting
• Antibody s suited for
  6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:20 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:50 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  van Muijen, G. N., Ruiter, D. J., Franke, W. W., Achtstatter, T., Haasnoot, W. H., Ponec, M., and Warnaar, S. O. (1986). Cell type heterogeneity of Cytokeratin expression in complex epithelia and carcinomas as demonstRated by monoclonal antibodies specific for Cytokeratins nos. 4 and 13, Exp Cell Res 162, 97-113. Weikel, W., Wagner, R., and Moll, R. (1987). Characterization of subcolumnar reserve cells and other epithelia of Human uterine cervix. DemonstRation of diverse Cytokeratin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline Cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with Keratin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102. Corver, W. E., Koopman, L. A., van der Aa, J., Regensburg, M., Fleuren, G. J., and Cornelisse, C. J. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intRatumor heterogeneity in cervical cancer, Cytometry 39, 96-107.
• Protein number
  P19013
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Cytokeratin   Keratin  
• Gene symbol
  KRT4
• Short name
  Mouse anti Cytokeratin 4 / Keratin K4
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Cytokeratin 4 / Keratin K4
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6441
• Gene
  KRT4
• Long gene name
  keratin 4
• Synonyms gene
  • CYK4
• Synonyms gene name
  • CYK4
• Synonyms
  • CK4
  • K4
• Synonyms name
  • cytokeratin 4
  • keratin, type II cytoskeletal 4
• Locus
  12q13.13
• Discovery year
  2001-06-22
• Entrez gene record
  3851
• Pubmed identfication
  • 16831889
• RefSeq identity
  • NM_002272
• Classification
  • Keratins, type II
• VEGA ID
  OTTHUMG00000169800

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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