Active AHCY, human recombinant

• Catalog number
  7527-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase
• Alternative_names
  SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase
• Description
  Catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy).
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  BioVision’s Active AHCY has been tested for its activity using Adenosyl Homocysteinase (AHCY) Activity Fluorometric Assay Kit (K807-100). It has a specific activity of >350 mU/mg.
• Results
  BioVision’s Active AHCY has been tested for its activity using Adenosyl Homocysteinase (AHCY) Activity Fluorometric Assay Kit (K807-100). It has a specific activity of >350 mU/mg.
• Unit Definition
  One unit of AHCY activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of adenosine/min at 37°C.
• Molecular Weight
  52.1 kDa (1-432 aa + N-terminal polyhistidine tag). In the native form the protein exists in the tetrameric form and this form is required for activity.
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  6 months
• Concentration
  5 mg/ml
• Appearance
  Liquid
• Physical form description
  5 mg/ml in 50 mM potassium phosphate, 1 mM EDTA, pH 7.2 containing 20% glycerol.
• Background Information
  AHCY (EC 3.3.1.1) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AHCY controls the intracellular S-adenosylhomocysteine (SAH) concentration that is crucial for transmethylation reactions. AHCY deficiency causes hypermethioninemia. The recombinant human AHCY produced in E.Coli is a single, non-glycosylated polypeptide chain and is purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   AHCY  
• Gene symbol
  AHCY
• Short name
  Active AHCY, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  functionnal AHCY, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:343
• Gene
  AHCY
• Long gene name
  adenosylhomocysteinase
• Synonyms gene name
  • S-adenosylhomocysteine hydrolase
• Synonyms
  • SAHH
• GenBank acession
  • M61832
• Locus
  20q11.22
• Discovery year
  1986-01-01
• Entrez gene record
  191
• Pubmed identfication
  • 7079734
  • 6580258
• RefSeq identity
  • NM_000687
• VEGA ID
  OTTHUMG00000140098

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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