MFGE8 Antibody

• Catalog number
  R31046
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MFGE8
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the MFGE8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MFGE8 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q08431
• Purity
  Antigen affinity
• Description
  Milk Fat Globule-Egf Factor 8, also called Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. It is a secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the gene to chromosome 15q25. Hanayama et al.(2002) found that it is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. When engaged by phospholipids, it bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that MFGE8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that the protein promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
• Immunogen
  An amino acid sequence from the middle region of human MFGE8 (NGHEFDFIHDVNKKHK) was used as the immunogen for this MFGE8 antibody.
• Storage
  After reconstitution, the MFGE8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MFGE8  
• Gene symbol
  MFGE8
• Short name
  Anti-MFGE8
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to MFGE8
• Alternative technique
  antibodies
• Alternative to gene target
  milk fat globule-EGF factor 8 protein, BA46 and EDIL1 and HMFG and hP47 and HsT19888 and MFG-E8 and MFGM and OAcGD3S and SED1 and SPAG10, MFGE8 and IDBG-28947 and ENSG00000140545 and 102723860,102724554,4240, phosphatidylethanolamine binding, Extracellular, Mfge8 and IDBG-193646 and ENSMUSG00000030605 and 17304, MFGE8 and IDBG-629001 and ENSBTAG00000003300 and 281913

Gene info

• Identity
  HGNC:7036
• Gene
  MFGE8
• Long gene name
  milk fat globule EGF and factor V/VIII domain containing
• Synonyms gene
  • SPAG10
• Synonyms gene name
  • sperm associated antigen 10
  • milk fat globule-EGF factor 8 protein
• Synonyms
  • SED1
  • EDIL1
  • BA46
  • OAcGD3S
  • HsT19888
  • MFG-E8
  • hP47
• Synonyms name
  • sperm surface protein hP47
  • lactadherin
• GenBank acession
  • U58516
• Locus
  15q26.1
• Discovery year
  1996-10-31
• Entrez gene record
  4240
• Pubmed identfication
  • 9027496
  • 19204935
• RefSeq identity
  • NM_005928
• VEGA ID
  OTTHUMG00000148682

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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