Protein L Sepharose Column

Description

Columns with Reusable Protein L conjugated Sepharose beads with ≥10 mg/ml binding capacity & high flow rate for antibody purification from multiple sources. Protein L-Sepharose is prepared by covalently coupling recombinant Protein L (contains five Ig light chain binding domains, BV catalog # 6530-10) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet bead.
Product Highlights

• CONTENTS: Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in 20 % Ethanol/H₂O. • FEATURES: Binding capacity of human IgG greater than 10 mg/ml of bead;High flow rate; Low falling off of rProteinA/G;pH stability 2-10. • APPLICATIONS: Purification of monoclonal and polyclonal antibodies.
Storage Temp

+4°C
Shipping

gel pack
Shelf Life

12 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.

Name

Chromatography
Concept

Scope note: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Tree numbers
- E05.196.181
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data