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Long name
Mouse Monoclonal Antibody against Human CD326 / EpCAM, Clone: VU-1D9
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Antigen
CD326 / EpCAM
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Species reactivity
Human (Homo sapiens), Other species Not tested
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
VU-1D9
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Applications
FC, IHC(P), IP, WB, IHC(F), ICC
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Immunogen
Small cell lung carcinoma cell line H69.
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Format conjugation
PerCP-Cy 5.5
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Specificity
The mouse monoclonal antibody VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
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Research area
CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD326 / EpCAM, Clone: VU-1D9 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C: EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 2008 Mar;61(3):307-10., *Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is _x000D_ a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48., *Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J: Flow cytometric analysis of antigen expression in malignant and normal renal cells. Anticancer Res. 2000 Jul-Aug;20(4):2773-8., *Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A: Immunohistochemical localization of epithelial glycoprotein EGP-2 and_x000D_ carcinoembryonic antigen in normal colonic mucosa and colorectal tumors. Anticancer Res. 1998 Sep-Oct;18(5B):3669-75., *Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S: Immunohistochemical demonstration of breast-derived and/or carcinoma-associated_x000D_ glycoproteins in normal skin appendages and their tumors. J Cutan Pathol. 1992 Feb;19(1):73-9._x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
