CTSB, mouse recombinant
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Catalog numberP1095-10
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PricePlease ask
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Size10 ug
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SynonymsCathepsin B, Ctsb, CB
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Alternative_namesCathepsin B, Ctsb, CB
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DescriptionAn anti-protease implicated in myoclonus epilepsy.
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RecombinantYes
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SourceInsect cell (Baculovirus)
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Endotoxin Level< 1 EU/ug
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Activity Specifications test methodMeasured by the hydrolysis of 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
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Biological activitySpecific activity is >2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
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Results>2,000 pmol/min/ug
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Unit DefinitionOne unit is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
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Molecular Weight36.4 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration0.5 mg/ml
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AppearanceLiquid
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Physical form descriptionIn Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
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Background InformationCTSB, also known as cathepsin B preproprotein, is a papain-family cysteine protease that is normally located in lysosomes. It has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Recombinant mouse CTSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Amino acid sequenceHDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCTSB
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Short nameCTSB, mouse recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesMouse, Mouses
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Alternative namecathepsin B, mouse Rec.
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Alternative techniquerec, murine
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Alternative to gene targetcathepsin B, APPS and CPSB, CTSB and IDBG-7654 and ENSG00000164733 and 1508, proteoglycan binding, nuclei, Ctsb and IDBG-172314 and ENSMUSG00000021939 and 13030, CTSB and IDBG-629395 and ENSBTAG00000012442 and 281105
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Gene info
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Identity
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Gene
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Long gene namecathepsin B
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cathepsins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data