Immunogen Purified human IgG
Purification Affinity purified goat IgG
Storage condition Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
Verified applications suitable for all immunoassays applications
Connected products antibodies to human immunoglobulins
Recommended dilutions for use optimal working dilution should be determined by the investigator
Verified reactivity human IgG Fc (two heavy chains with constant domains)
Possible reactivity human IgG Fc (two heavy chains with constant domains)
No reactivity no confirmed exceptions from predicted reactivity known in the moment
Supplementary information This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains, non-immunoglobulin human serum proteins or IgG F(ab)'2 based on immunoelectrophoresis.
References to be added when available
Scientific context Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Notes biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name Capra aegagrus hircus
Short name Goat, Fc, conjugated
Technique Goat, FC
Alternative name caprine, fragment c, biotinilated coupled
Alternative technique caprine