Goat, Fc, Biotin conjugated
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Catalog number
AS10 788
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Price
Please ask
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Size
2 mg
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Available ordering format
Liquid
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Immunogen
Purified human IgG
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
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Verified applications
suitable for all immunoassays applications
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Connected products
antibodies to human immunoglobulins
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Recommended dilutions for use
optimal working dilution should be determined by the investigator
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human IgG Fc (two heavy chains with constant domains)
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Possible reactivity
human IgG Fc (two heavy chains with constant domains)
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains, non-immunoglobulin human serum proteins or IgG F(ab)'2 based on immunoelectrophoresis.
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References
to be added when available
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Scientific context
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
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Notes
biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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Gene target
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Short name
Goat, Fc, conjugated
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Technique
Goat, FC, antibody Conjugates, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
Biotin
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Species
Goats
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Alternative name
caprine, fragment c, biotinilated coupled
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Alternative technique
caprine
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