Luciferase Antibody
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Catalog number3858-200
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PricePlease ask
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Size200 µg
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Antibody TargetLuciferase
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Target Alternative NameChinese Firefly luciferase
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Antibody TypePolyclonal
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Species ReactivitiesLuciferase
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Immunogen SequenceRecombinant Chinese Firefly luciferase
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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FormulationIn phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
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PurificationAffinity purified
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ApplicationWestern blot
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Positive ControlChinese Firefly luciferase (Cat.# 7901-150)
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Application And UsagesWestern blotting (0.5-4 μg/ml). However, the optimal conditions should be determined individually. The antibody recognizes Chinese firefly luciferase. Cross-reactivity to other types of Luciferases have not been determined. Recombinant Chinese firefly luciferase (Cat.# 7901-5) can be used as a positive control.
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DescriptionThe Firefly luciferase is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis endemic to Yunnan province, China. It has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis as well as a broader and more physiologically relevant effective pH range. Luciferase has been used as a sensitive reporter for studying gene regulation and function. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameLuciferase Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Labelluciferase
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Alternative nameLuciferase (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data