Anti- Histone H2AZ, Rabbit Monoclonal Antibody
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Catalog number
A1142-25
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Price
Please ask
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Size
25 µg
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Antibody Target
H2AFZ
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Target Alternative Name
Histone H2A.Z, H2A/z, H2AFZ
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Antibody Type
Monoclonal
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Clone
RM215
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Species Reactivities
Human
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Immunogen Sequence
A peptide corresponding to the C-terminus of human Histone H2AZ
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Accession
P0C0S5
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Gene Id
3015
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Purification
Affinity purified using protein A
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Application
WB, ICC, ELISA, Multiplex
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Positive Control
WB: HeLa, HEK293, A375, SK-MEL-2 and A431; ICC: Hela cells
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Application And Usages
WB: 0. 1 µg/mL – 0.5 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.1 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL.
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Description
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
H2AZ1
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Short name
Anti- Histone H2AZ, Rabbit Monoclonal Antibody
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Technique
Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Rabbit, Rabbits
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Isotype
Rabbit IgG
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Alternative name
antibody to- Histone H2AZ, production species: rabbit monoclonal (antibody to-)
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Alternative technique
antibodies, rabbit-anti
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Gene info
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Identity
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Gene
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Long gene name
H2A.Z variant histone 1
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Synonyms gene
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Synonyms gene name
- H2A histone family, member Z
- H2A histone family member Z
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Synonyms
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GenBank acession
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Locus
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Discovery year
1993-10-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product files
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