Phospho-SMAD2 Antibody (pS118)
-
Catalog number
F48605-0.4ML
-
Price
Please ask
-
Size
0.4 ml
-
-
Category
Antibody
-
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
-
Form
Antigen affinity purified
-
Conjugation
Unconjugated
-
Clone
Polyclonal antibody
-
Recognised antigen
Phospho-SMAD2 (pS118)
-
Host animal
Rabbit (Oryctolagus cuniculus)
-
Clonality
Polyclonal (rabbit origin)
-
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applications
IF, DB, ELISA
-
Recommended dilutions
Immunofluorescence: 1:10-1:50,Dot blot: 1:500
-
Notes
Titration of the phospho-SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented use
This phospho-SMAD2 antibodyis to be used only for research purposes and not for diagnostics..
-
Uniprot
Q15796
-
Purity
Antigen affinity
-
Description
The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
-
Immunogen
This phospho-SMAD2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS118 of human SMAD2.
-
Storage
Aliquot the phospho-SMAD2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Gene symbol
SMAD2
-
Short name
Anti-Phospho-SMAD2 (pS118)
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Rabbit Ig
-
Alternative name
Antibodies to Phospho-SMAD2 (pS118)
-
Alternative technique
antibodies
-
Alternative to gene target
SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010
-
Gene info
-
Identity
-
Gene
-
Long gene name
SMAD family member 2
-
Synonyms gene
-
Synonyms gene name
- MAD, mothers against decapentaplegic homolog 2 (Drosophila)
- SMAD, mothers against DPP homolog 2 (Drosophila)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
1996-11-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products