HA-Tag Antibody (Clone 12CA5[2])

• Catalog number
  3996-200
• Price
  Please ask
• Size
  200 µg

• Antibody Target
  HA-Tag
• Target Alternative Name
  GRIN2A , hNR2A , NMDAR2A , NR2A
• Antibody Type
  Monoclonal
• Clone
  12CA5[2]
• Species Reactivities
  All species
• Immunogen Sequence
  Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein
• Accession
  ADG21028
• Gene Id
  295441961
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1.0 mg/ml
• Formulation
  200 µg (1 mg/ml) anti-HA-Tag monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation, Immunofluorescence
• Application And Usages
  Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
• Description
  Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HA-Tag   12CA5[2]  
• Gene symbol
  MYO1G, KRT31, KRT32, KRT33B, KRT34, KRT35, ARHGAP45, KRT33A, AKAP13, PUM3, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2, SNORD116-2
• Short name
  HA-Tag Antibody (Clone 12CA5[2])
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Isotype
  Mouse IgG2b
• Label
  tag
• Alternative name
  HA-detection labelled (antibody to-) (clonality 12CA5[2])
• Alternative technique
  antibodies
• Clone name
  12CA5

Gene info

• Identity
  HGNC:13880
• Gene
  MYO1G
• Long gene name
  myosin IG
• Synonyms
  • HA-2
• Synonyms name
  • minor histocompatibility antigen HA-2
• GenBank acession
  • AF380932
• Locus
  7p13
• Discovery year
  2000-11-28
• Entrez gene record
  64005
• Classification
  • Myosin heavy chains, class I
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000155821

Gene info

• Identity
  HGNC:6448
• Gene
  KRT31
• Long gene name
  keratin 31
• Synonyms gene
  • KRTHA1
• Synonyms gene name
  • keratin, hair, acidic, 1
  • keratin 31, type I
• Synonyms
  • Ha-1
• Synonyms name
  • hard keratin type I 1
• GenBank acession
  • X86570
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3881
• Pubmed identfication
  • 7578244
  • 16831889
• RefSeq identity
  • NM_002277
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133423

Gene info

• Identity
  HGNC:6449
• Gene
  KRT32
• Long gene name
  keratin 32
• Synonyms gene
  • KRTHA2
• Synonyms gene name
  • keratin, hair, acidic, 2
  • keratin 32, type I
• Synonyms
  • Ha-2
• Synonyms name
  • hard keratin type I
• GenBank acession
  • X90761
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3882
• Pubmed identfication
  • 7556444
  • 8823373
  • 16831889
• RefSeq identity
  • NM_002278
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133430

Gene info

• Identity
  HGNC:6451
• Gene
  KRT33B
• Long gene name
  keratin 33B
• Synonyms gene
  • KRTHA3B
• Synonyms gene name
  • keratin, hair, acidic, 3B
  • keratin 33B, type I
• Synonyms
  • Ha-3II
• Synonyms name
  • hard keratin type I 3II
• GenBank acession
  • X82634
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3884
• Pubmed identfication
  • 7565656
  • 16831889
• RefSeq identity
  • NM_002279
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133429

Gene info

• Identity
  HGNC:6452
• Gene
  KRT34
• Long gene name
  keratin 34
• Synonyms gene
  • KRTHA4
• Synonyms gene name
  • keratin, hair, acidic, 4
  • keratin 34, type I
• Synonyms
  • Ha-4
• Synonyms name
  • hard keratin type I 4
• GenBank acession
  • Y16790
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3885
• Pubmed identfication
  • 2431943
  • 9756910
  • 16831889
• RefSeq identity
  • NM_021013
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133436

Gene info

• Identity
  HGNC:6453
• Gene
  KRT35
• Long gene name
  keratin 35
• Synonyms gene
  • KRTHA5
• Synonyms gene name
  • keratin, hair, acidic, 5
  • keratin 35, type I
• Synonyms
  • Ha-5
• Synonyms name
  • hard keratin type I 5
• GenBank acession
  • X90762
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3886
• Pubmed identfication
  • 8823373
  • 16831889
• RefSeq identity
  • NM_002280
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133425

Gene info

• Identity
  HGNC:17102
• Gene
  ARHGAP45
• Long gene name
  Rho GTPase activating protein 45
• Synonyms gene
  • HMHA1
• Synonyms gene name
  • histocompatibility (minor) HA-1
• Synonyms
  • KIAA0223
  • HA-1
• GenBank acession
  • D86976
• Locus
  19p13.3
• Discovery year
  2005-12-06
• Entrez gene record
  23526
• Pubmed identfication
  • 9820596
  • 9039502
• Classification
  • Minor histocompatibility antigens
  • AH/BAR family Rho GTPase activating proteins
  • F-BAR domain containing

Gene info

• Identity
  HGNC:6450
• Gene
  KRT33A
• Long gene name
  keratin 33A
• Synonyms gene
  • KRTHA3A
• Synonyms gene name
  • keratin, hair, acidic, 3A
  • keratin 33A, type I
• Synonyms
  • Ha-3I
  • Krt1-3
• Synonyms name
  • hard keratin type I 3I
• GenBank acession
  • Y16788
• Locus
  17q21.2
• Discovery year
  2004-10-20
• Entrez gene record
  3883
• Pubmed identfication
  • 7565656
  • 16831889
• RefSeq identity
  • NM_004138
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133432

Gene info

• Identity
  HGNC:371
• Gene
  AKAP13
• Long gene name
  A-kinase anchoring protein 13
• Synonyms gene
  • LBC
• Synonyms gene name
  • lymphoid blast crisis oncogene
  • A kinase (PRKA) anchor protein 13
• Synonyms
  • Ht31
  • BRX
  • AKAP-Lbc
  • c-lbc
  • PROTO-LB
  • HA-3
  • ARHGEF13
• GenBank acession
  • M90360
• Locus
  15q25.3
• Discovery year
  1999-09-16
• Entrez gene record
  11214
• Pubmed identfication
  • 9627117
  • 1860836
• RefSeq identity
  • NM_007200
• Classification
  • A-kinase anchoring proteins
  • Dbl family Rho GEFs
  • Minor histocompatibility antigens
  • Pleckstrin homology domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000172205

Gene info

• Identity
  HGNC:29676
• Gene
  PUM3
• Long gene name
  pumilio RNA binding family member 3
• Synonyms gene
  • KIAA0020
• Synonyms gene name
  • KIAA0020
• Synonyms
  • XTP5
  • PEN
  • PUF6
  • hPUF-A
  • HA-8
  • Puf-A
• Synonyms name
  • penguin homolog (Drosophila)
  • minor histocompatibility antigen HA-8
• GenBank acession
  • AL832239
• Locus
  9p24.2
• Discovery year
  2004-01-06
• Entrez gene record
  9933
• Pubmed identfication
  • 7584026
  • 7584028
  • 21266351
• RefSeq identity
  • NM_014878
• Classification
  • Pumilio homology domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000019450

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:34270
• Gene
  RNU6-2
• Long gene name
  RNA, U6 small nuclear 2
• Synonyms
  • U6-2
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  103625684
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_125730
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:18923
• Gene
  KRTAP13-2
• Long gene name
  keratin associated protein 13-2
• Synonyms
  • KAP13-2
• GenBank acession
  • AP001708
• Locus
  21q22.11
• Discovery year
  2003-02-21
• Entrez gene record
  337959
• Pubmed identfication
  • 12359730
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057793

Gene info

• Identity
  HGNC:32990
• Gene
  SNORD114-2
• Long gene name
  small nucleolar RNA, C/D box 114-2
• Synonyms
  • 14q(II-2)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767578
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003194.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33068
• Gene
  SNORD116-2
• Long gene name
  small nucleolar RNA, C/D box 116-2
• Synonyms
  • HBII-85-2
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033414
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003317
• Classification
  • Small nucleolar RNAs, C/D box

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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