Anti-Mouse CD25, Biotin (Clone PC61.5.3) (rat IgG1)

• Catalog number
  MBS520077
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [CD25]
• Products_name_syn
  [CD25, Biotin (Clone PC61.5.3) (rat IgG1); Biotin Anti-Mouse CD25 (IL-2R) Monoclonal Antibody]
• Other_names
  [CD25; Peripheral myelin protein 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein]
• Products_gene_name
  [CD25]
• Other_gene_names
  [Pmp22; Cd25; Pmp-22; PMP-22; SAG]
• Clonality
  Monoclonal
• Clone
  [PC61.5.3]
• Reactivity
  Mouse
• Specificity
  CD25 (IL-2Ra, p55, Ly 43) (Mouse CD25 (IL-2 receptor, a chain))
• Form
  Biotin (Biotin conjugated purified Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ ml. (Purified from ascitic fluid via Protein G Chromatography))
• Storage_stability
  Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
• Tested_application
  Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  CD25   PC61   IgG1  
• Gene symbol
  ISG20, IL2RA
• Short name
  Anti-Mouse CD25, (Clone PC61 5 3) ( IgG1)
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Rat
• Isotype
  Rat IgG1, IgG1
• Label
  Biotin
• Species
  Rat, Mouses, Rats
• Alternative name
  Antibody toMouse CD25, biotinilated (clonality PC61.5.3) (rat IgG1)
• Alternative technique
  murine, antibodies
• Clone name
  PC61

Gene info

• Identity
  HGNC:6130
• Gene
  ISG20
• Long gene name
  interferon stimulated exonuclease gene 20
• Synonyms gene name
  • interferon stimulated exonuclease gene 20kDa
• Synonyms
  • HEM45
  • CD25
• GenBank acession
  • X89773
• Locus
  15q26.1
• Discovery year
  1998-01-30
• Entrez gene record
  3669
• Pubmed identfication
  • 9235947
  • 9605874
• RefSeq identity
  • NM_002201
• Classification
  • Exonucleases
• VEGA ID
  OTTHUMG00000148679

Gene info

• Identity
  HGNC:6008
• Gene
  IL2RA
• Long gene name
  interleukin 2 receptor subunit alpha
• Synonyms gene
  • IL2R
  • IDDM10
• Synonyms gene name
  • insulin-dependent diabetes mellitus 10
  • interleukin 2 receptor, alpha
• Synonyms
  • CD25
• GenBank acession
  • X01057
• Locus
  10p15.1
• Discovery year
  1990-01-22
• Entrez gene record
  3559
• Pubmed identfication
  • 3925551
  • 17676041
• RefSeq identity
  • NM_000417
• Classification
  • CD molecules
  • Sushi domain containing
  • Interleukin receptors
• VEGA ID
  OTTHUMG00000017616
• Locus Specific Databases
  • IL2RAbase: Mutation registry for Interleuken 2 receptor α deficiency
  • LRG_73

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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