Phospho-Stat6 Antibody
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Catalog number3476-100
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PricePlease ask
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Size100 µg
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Antibody TargetPhospho-STAT6
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Target Alternative NameSTAT6B , STAT6C , D12S1644 , IL-4-STAT , interleukin 4 induced STAT
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse
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Immunogen SequenceSynthetic phospho-peptide corresponding to resides surrounding Tyr641 of human Stat6
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AccessionNP_003144
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Gene Id6778
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide. The antibody recognizes ~110 kDa phosphorylated Stat6 (Tyr641) of human and mouse origins. Reactivity to other species has not been tested.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (1-4 µg/ml) and Immunohistochemistry (20 µg/ml). However, the optimal concentrations should be determined individually.
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DescriptionMembrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Althoμgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short namePhospho-Stat6 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namephosphorilated-signal transducer and activator on transcription 6, interleukin-4 induced (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetsignal transducer and activator of transcription 6, interleukin-4 induced, D12S1644 and IL-4-STAT and STAT6B and STAT6C, STAT6 and IDBG-42122 and ENSG00000166888 and 6778, sequence-specific DNA binding, nuclei, Stat6 and IDBG-195602 and ENSMUSG00000002147 and 20852, STAT6 and IDBG-635695 and ENSBTAG00000006335 and 353105
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data