This antibody has been succesfully tested for use in following methods:
Western blot, IF.
Target of this antibody:
Formulation of this antibody:
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Alternative names of antibody target:
Western Blot: 1:1000, IF: 1:500
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Protein G purified
Polyclonal antibody raised in rabbit against human HP1α (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein.
How to handle this antibody:
The antibody solution should be gently mixed before use.
CBX5/HP1 alpha is a component of heterochromatin. It recognizes and binds to histone H3 tails methylated at ‘Lys-9’, leading to epigenetic repression of transcription. HP1 alpha may also interact with lamin B receptor (LBR), thereby contributing to the association of the heterochromatin with the inner nuclear membrane. Further, HP1 alpha is involved in the formation of a functional kinetochore through interaction with MIS12 complex proteins.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anti-HP1α polyclonal antibody
Polyclonal, Antibody, anti
antibody to-HP1α polyclonal (Antibody to)