NPM (phospho-T199) polyclonal antibody
-
Catalog number
AP0100-50ul
-
Price
Please ask
-
Size
50ul
-
-
Alternative Name
Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM
-
Immunogen
Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 199.
-
Swiss Prot
P06748
-
Reactivity
Human,Mouse,Rat
-
Applications
WB
-
Application_all
WB: 1:500~1:1000
-
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 97% (by SDS-PAGE).
-
Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
Specificity
p-NPM (T199) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr199.
-
Usage
Western blot (WB) analysis of p-NPM (T199) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:CT26 whole cell lysate(40ug) Lane7:HEK293T whole cell lysate(40ug)
-
BiowMW
~ 38 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
NPM1
-
Short name
NPM (phospho-T199) polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit
-
Alternative name
NPM (phosphorilated-T199) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene name
nucleophosmin 1
-
Synonyms gene name
- nucleophosmin (nucleolar phosphoprotein B23, numatrin)
- nucleophosmin
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1993-08-24
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products