E-selectin antibody (FITC)
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Catalog number
GEN833588
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Price
Please ask
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Size
100 Tests
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Also known as
E-selectin (FITC)
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Other names
E-selectin; E-selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; selectin E; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1; ELAM-1; LECAM2
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Gene name synonims
SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1; ELAM-1; LECAM2
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Other gene names
SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1; ELAM-1; LECAM2
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
CI26CI0B7
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Host organism
Mouse (Mus musculus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Human E-selectin; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
> 95% as determined by SDS-Page
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Form Appearance
Supplied in 50 mM TRIS, 100 mM NaCl, 1% BSA, 0.02% NaN3, pH 7.4
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Concentration
N/A
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Storage and shipping
For short term storage keep the antibody at +4°C. Variations in the range 1°C to 9°C are tolerable. For extended periods of time aliquote keep the antibody at -20°C. Cycles of freeze/defrost may denaturate the antibodies, thus reducing their activity..
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Tested applications
ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Paraffin), Western Blot (WB)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
SELE, CD44
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Short name
E-selectin antibody (FITC)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Anti-E-selectin antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
selectin E
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Synonyms gene
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Synonyms gene name
- endothelial adhesion molecule 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
- CD molecules
- Sushi domain containing
- Selectins
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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