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Antibody Target
MAPK14
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Target Alternative Name
p38MAPK, p38-MAPK, p38 Mitogen Activated Protein Kinase, Mxi2, MAX-interacting protein 2, CSBP1, CSBP2, CSPB1, Csaids ,APK2A, stress-activated protein kinase 2A, p38, p38 Alpha, MAPK14, Mitogen Activated Protein Kinase 14, Exip, PRKM14, PRKM15, RK
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Antibody Type
Monoclonal
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Clone
RM243
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Species Reactivities
Human
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Immunogen Sequence
A phospho-peptide corresponding to human Phospho-p38 MAPK (Thr180/Tyr182)
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Accession
Q16539 , Q15759 , O15264 , P53778
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Gene Id
1432 , 5600 , 5603 , 6300
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Purification
Affinity purified using protein A
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Application
IHC, WB
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Positive Control
WB: Hela cell lysate; IHC: human breast cancer tissue
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Application And Usages
WB: 1:1000 -1:2000 dilution; IHC: 1:500 - 1:1000 dilution
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Description
p38 MAP kinase is the mammalian homologue of the yeast HOG kinase and participates in a cascade controlling cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including inflammatory cytokines, UV light and growth factors, etc. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps