Anti- His-Tag Rabbit Monoclonal Antibody
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Catalog number
A1138-50
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Price
Please ask
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Size
50 µg
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Antibody Target
His
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Target Alternative Name
His-Tag
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Antibody Type
Monoclonal
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Clone
RM146
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Immunogen Sequence
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Purification
Affinity purified using protein A
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Application
IP, WB, ICC, FC, IHC
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Positive Control
WB: 293T cell lysate; IP: 293T cells; IHC: HepG2 cells; ICC: 293T cells
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Application And Usages
Western Blot: 0.1 ug/mL – 1 ug/mL;Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL.
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Description
A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
histidine
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Anti- His-Tag Rabbit Monoclonal Antibody
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Technique
Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Host
Rabbit, Rabbits
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Isotype
Rabbit IgG
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Label
His
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Alternative name
antibody to- histidine-detection labelled production species: rabbit monoclonal (antibody to-)
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Alternative technique
antibodies, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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