Mouse H-2 class I histocompatibility antigen, Q7 alpha chain ELISA Kit
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Catalog numberEK3889
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PricePlease ask
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Size96-wells plate (breakable strips)
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Long nameELISA test kit for Mouse H-2 class I histocompatibility antigen, Q7 alpha chain
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Synonym nameMurine H-2 class I histocompatibility antigen, Q7 alpha chain ELISA Kit
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General descriptionThis is a ready to use kit for performing ELISA tests for detection of H-2 class I histocompatibility antigen, Q7 alpha chain in samples from Mouse
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AntigenH-2 class I histocompatibility antigen, Q7 alpha chain
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Species reactivityMouse
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Sample typesOur H-2 class I histocompatibility antigen, Q7 alpha chain ELISA kit has been proven to work and successfully detect H-2 class I histocompatibility antigen, Q7 alpha chain in Murine
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Kit contentsELISA test kit for Mouse H-2 class I histocompatibility antigen, Q7 alpha chainAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
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Detection wavelengt450 nm
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Assay procedure1-4h
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Summary of the prodecure1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
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ApplicationsEnzyme Linked Immunosorbent Assay
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Shipping storage shelf lifeThe H-2 class I histocompatibility antigen, Q7 alpha chain ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Mouse H-2 class I histocompatibility antigen, Q7 alpha chain ELISA Kit is 6 months after delivery.
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TipsDue to transportation and handling small amounts of the liquid components of the Murine H-2 class I histocompatibility antigen, Q7 alpha chain ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
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DescriptionThe H-2 class I histocompatibility antigen, Q7 alpha chain ELISA is a α- or alpha protein sometimes glycoprotein present in blood. Antigens are peptides or recombinant or native dependent on the production method.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolNCAPH2, SNAR-H, PFDN6, TUBA3D, NCAPH, NME2, HLA-H, CFB, IGHV3-69-1, FUT1
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Short nameH-2 class I histocompatibility antigen, Q7 alpha chain ELISA
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TechniqueELISA kit, antigen, ELISA, Mouse, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Hostmouse, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesMouse, Mouses
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Alternative nameMouse H-2 class I histocompatibility protein, Q7 a epitope Enzyme-linked immunosorbent assay reagent
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Alternative techniqueantigenes, murine, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namenon-SMC condensin II complex subunit H2
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Synonyms gene name
- non-SMC condensin II complex, subunit H2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-09-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Condensin II subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA H
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Synonyms gene name
- small ILF3/NF90-associated RNA H
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2008-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene nameprefoldin subunit 6
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Synonyms gene
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Synonyms gene name
- HLA class II region expressed gene KE2
- prefoldin 6
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-06-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Prefoldin subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametubulin alpha 3d
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tubulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenon-SMC condensin I complex subunit H
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Synonyms gene
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Synonyms gene name
- barren (Drosophila) homolog
- barren homolog (Drosophila)
- barren homolog 1 (Drosophila)
- non-SMC condensin I complex, subunit H
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Condensin I subunits
- Kleisins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNME/NM23 nucleoside diphosphate kinase 2
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Synonyms gene name
- non-metastatic cells 2, protein (NM23B) expressed in
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NME/NM23 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, H (pseudogene)
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GenBank acession
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Locus
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Discovery year1991-08-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecomplement factor B
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Synonyms gene
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Synonyms gene name
- B-factor, properdin
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-69-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 3-H (provisional)
- immunoglobulin heavy variable 3-H pseudogene (provisional)
- immunoglobulin heavy variable 3-H (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data