igScript™ First Strand cDNA Synthesis Kit - 25 Reactions

Description

igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55 ºC. Product Includes: 5x igScript™ master mix Oligo d(T)23 VN primer (50 µM) Random hexamer primer mix (60 µM) Nuclease free water
Shipping Condition

Ships with Dry Ice
Additional description

this 1 cDNA is double stranded complementary DNA derived by Intact Genomics from mRNA with very high fidelity reverse transcription using a pfu or pfx mix

Gene target

igScript™ First Strand Synthesis Kit
Short name

igScript™ First Strand cDNA Synthesis Kit - 25
Technique

cDNA, cDNAs
Alternative name

igScript™ First Strand complementary Desoxyribonucleic acid Synthesis reagent - 25 Reactions
Alternative technique

pcr, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

cdna