LOX, Lipoxygenase (Affinity purified)
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Catalog numberAS06 128A
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PricePlease ask
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Size50 µg
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Available ordering formatLyophilized PBS pH 7.4
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Immunogennative lipoxygenase, type I-B, purified from Glycine max (Sigma, product number L7395) P08170
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteFor reconstitution of lyophilized unit please add 50 µl of sterile water.
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB)
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Connected productsAS06 128 | anti-LOX | lipoxygenase, rabbit antibodies (not purified serum)AS07 258 | anti-LOX-C | chloroplastic lipoxygenaseAS08 340 | anti-LOX2 | lipoxygenase 2 proteinPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use1:5000 (WB)
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Molecular weight expected аpparent54 (subunit), 108 (native enzyme)
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Verified reactivityArabidopsis thaliana, Glycine max
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Possible reactivityLathyrus undulatus, Malus x domestica, Solanum tuberosum
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationContact Gentaur support center at [email protected]
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Referencesto be added when available, antibody released in December 2014
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Scientific contextLipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals).Synonymes: linoleate, oxygen oxidoreductase.
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NotesNone
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Protein numberP08170
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TAIR numberRefer to NCBI
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TestA high affinity purification column was use to purify LOX, Lipoxygenase ( ) by agisera by chromatographic size exclusion.
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Description1 of protein A or G purified by agisera will give more specificity than crude serum.
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Gene target
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Gene symbolLOX, ALOX5, ALOX12B, ALOX12, ALOX15, ALOX15B, ALOXE3, OLR1
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Short nameLOX, Lipoxygenase ( )
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Techniqueaffinity, purified, purifications
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Alternative nameLOX, Lipoxygenase (protein enriched)
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Alternative techniquepure
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Gene info
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Identity
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Gene
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Long gene namelysyl oxidase
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namearachidonate 5-lipoxygenase
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-10
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearachidonate 12-lipoxygenase, 12R type
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-07-22
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namearachidonate 12-lipoxygenase, 12S type
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Synonyms gene name
- arachidonate 12-lipoxygenase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-08-07
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearachidonate 15-lipoxygenase
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-20
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearachidonate 15-lipoxygenase type B
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Synonyms gene name
- arachidonate 15-lipoxygenase, second type
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-22
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearachidonate lipoxygenase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-11-29
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Entrez gene record
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Pubmed identfication
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Classification
- Arachidonate lipoxygenases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameoxidized low density lipoprotein receptor 1
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Synonyms gene name
- oxidised low density lipoprotein (lectin-like) receptor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
- Scavenger receptors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
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Tree numbers
- E05.393.220
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Qualifiersdrug effects, methods, radiation effects